| 1. | Laser confocal fluorescence microscopy
激光共聚焦荧光显微镜 |
| 2. | Twophoton laser scanning fluorescence microscopy and its applications
双光子激光扫描荧光显微镜及其应用 |
| 3. | Fluorescence microscopy a form of microscopy in which fluorescent probes are added to the material being investigated
荧光显微术：用来观察研究插入荧光探针后的物体的一种显微技术。 |
| 4. | Mycobacteria can also be stained with auramine and viewed with fluorescence microscopy , in which acid fast bacilli now appear as glowing yellow rods
分枝杆菌也能被金胺染色，荧光显微镜下抗酸杆菌为发黄*色荧光的杆菌。 |
| 5. | Biofilm containing srb was observed on the surface of 316l ss by fluorescence microscopy in medicated postgate ' s c medium inoculated srb
荧光显微镜观察发现，在接种srb的修正的postgate ' sc培养基中， srb在316lss表面附着发展并形成生物膜。 |
| 6. | After 12h - long transport , 6 ( 5 ) cf could be detected in the stock by using fluorescence microscopy . the result of transport was accord with the histological observation
证实该时期6 ( 5 ) cf可以自接穗跨过嫁接面向砧木运输，这与组织学观察结果一致。 |
| 7. | For the first time , we observed the transformation of the j - aggregates morphologies depending on the surrounding humidity by fluorescence microscopy and atomic force microscopy
利用荧光显微镜和原子力显微镜我们第一次观察到j -聚集体的形貌随着湿度的变化而变化。 |
| 8. | Through many years " hard work , we successfully developed the first commercial laser confocal scanning fluorescence microscopy and the first gene - chip scanner in our country
通过多年的努力奋斗，成功地开发出国内首台商品化的激光共聚焦扫描荧光显微镜，并且成功开发出国内首台基因芯片扫描仪。 |
| 9. | In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection
此外，为了得到可溶性重组eo蛋白并便于观察重组蛋白的表达情况，我们将egfp基因与eo基因相连插入昆虫杆状病毒转移载体中，与线性杆状病毒dna共转染sf9细胞后通过噬斑纯化得到纯的重组杆状病毒，将其感染sf9细胞制备p1种子液，同时用荧光显微镜观察绿色荧光蛋白的表达情况剔除表达效果差的重组杆状病毒。再用p1种子液感染sf9细胞制备高效价的p2种子液。通过病毒液的梯度稀释和噬斑测定，确定p2种子液的病毒滴度达1 . 14 10 ~ 7pfu ml 。 |
